Recombinant Mouse Ppie Protein, Myc/DDK-tagged

• Cat.No.: Ppie-5042M
• Product Overview: Purified recombinant protein of mouse full-length peptidylprolyl isomerase E (cyclophilin E) (Ppie), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 33.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ppie peptidylprolyl isomerase E (cyclophilin E) [ Mus musculus (house mouse) ]
• Official Symbol: Ppie
• Synonyms: PPIE; peptidylprolyl isomerase E (cyclophilin E); peptidyl-prolyl cis-trans isomerase E; PPIase E; rotamase E; cyclophilin E; cyclophilin-33; Cyp33; 2010010D16Rik
• Gene ID: 56031
• mRNA Refseq: NM_019489
• Protein Refseq: NP_062362
• UniProt ID: Q9QZH3