Recombinant Mouse Polr3c Protein, Myc/DDK-tagged

• Cat.No.: Polr3c-5004M
• Product Overview: Purified recombinant protein of mouse full-length polymerase (RNA) III (DNA directed) polypeptide C (Polr3c), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF-Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner.
• Molecular Mass: 61.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Polr3c polymerase (RNA) III (DNA directed) polypeptide C [ Mus musculus (house mouse) ]
• Official Symbol: Polr3c
• Synonyms: POLR3C; polymerase (RNA) III (DNA directed) polypeptide C; DNA-directed RNA polymerase III subunit RPC3; RNA polymerase III subunit C3; DNA-directed RNA polymerase III subunit C; RPC3; RPC62; 4933407E01Rik
• Gene ID: 74414
• mRNA Refseq: NM_028925
• Protein Refseq: NP_083201
• UniProt ID: Q9D483