Recombinant Mouse Plekhg6 Protein, Myc/DDK-tagged

• Cat.No.: Plekhg6-4930M
• Product Overview: Purified recombinant protein of mouse full-length pleckstrin homology domain containing, family G (with RhoGef domain) member 6 (Plekhg6), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis.
• Molecular Mass: 89.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plekhg6 pleckstrin homology domain containing, family G (with RhoGef domain) member 6 [ Mus musculus (house mouse) ]
• Official Symbol: Plekhg6
• Synonyms: PLEKHG6; pleckstrin homology domain containing, family G (with RhoGef domain) member 6; pleckstrin homology domain-containing family G member 6; PH domain-containing family G member 6; Gm155; BC026778; MGC30363
• Gene ID: 213522
• mRNA Refseq: NM_198604
• Protein Refseq: NP_941006
• UniProt ID: Q8R0J1