Recombinant Mouse Plce1 Protein, Myc/DDK-tagged

• Cat.No.: Plce1-4916M
• Product Overview: Purified recombinant protein of mouse full-length phospholipase C, epsilon 1 (Plce1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation. In podocytes, is involved in the regulation of lamellipodia formation. Acts downtream of AVIL to allow ARP2/3 complex assembly.
• Molecular Mass: 255 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plce1 phospholipase C, epsilon 1 [ Mus musculus (house mouse) ]
• Official Symbol: Plce1
• Synonyms: Plce1; phospholipase C, epsilon 1; PL; Plce; mKIAA1516; PLCepsilon; 4933403A21Rik; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; PLC-epsilon-1; phosphoinositide phospholipase C-epsilon-1; EC 3.1.4.11
• Gene ID: 74055
• mRNA Refseq: NM_019588
• Protein Refseq: NP_062534
• UniProt ID: Q8K4S1