Recombinant Mouse Plat protein, His-tagged

• Cat.No.: Plat-2739M
• Product Overview: Recombinant Mouse Plat aa. (Glu359~Leu532 (Accession # P11214)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Glu359~Leu532
• Description: This gene encodes a key enzyme of the fibrinolytic pathway. The encoded protein undergoes proteolytic processing by plasmin to generate a heterodimeric serine protease that cleaves the proenzyme plasminogen to produce plasmin, a protease that is required to break down fibrin clots. Additionally, the encoded protein is involved in other biological processes such as synaptic plasticity, cell migration and tissue remodeling. Mice lacking the encoded protein display a reduction in long-term potentiation in hippocampus and conversely, transgenic mice overexpressing the encoded protein have increased and prolonged long-term potentiation.
• Form: Freeze-dried powder
• Molecular Mass: 21 kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Plat plasminogen activator, tissue [ Mus musculus (house mouse) ]
• Official Symbol: Plat
• Synonyms: tPA; AU020998; AW212668; D8Ertd2e; t-plasminogen activator; tissue-type plasminogen activator
• Gene ID: 18791
• mRNA Refseq: NM_008872.3
• Protein Refseq: NP_032898.2
• UniProt ID: P11214