Recombinant Mouse Pla2g2f Protein, Myc/DDK-tagged

• Cat.No.: Pla2g2f-4900M
• Product Overview: Purified recombinant protein of mouse full-length phospholipase A2, group IIF (Pla2g2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases. Phospholipase A2, which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Hydrolyzes phosphatidylethanolamine more efficiently than phosphatidylcholine, with only a modest preference for arachidonic acid versus linoelic acid at the sn-2 position. Comparable activity toward 1-palmitoyl-2-oleoyl-phosphatidylserine vesicles to that toward 1-palmitoyl-2-oleoyl-phosphatidylglycerol. Prefers phosphatidylglycerol compared to phosphatidylcholine.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 23.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pla2g2f phospholipase A2, group IIF [ Mus musculus (house mouse) ]
• Official Symbol: Pla2g2f
• Synonyms: Pla2g2f; phospholipase A2, group IIF; mGII; GIIFsPLA2; sPLA2-IIF; group IIF secretory phospholipase A2; GIIF sPLA2; phosphatidylcholine 2-acylhydrolase 2F; EC 3.1.1.4
• Gene ID: 26971
• mRNA Refseq: NM_012045
• Protein Refseq: NP_036175
• UniProt ID: Q9QZT4