Recombinant Mouse Pkm Protein, His-tagged

Cat.No. : Pkm-968M
Product Overview : Recombinant mouse PKM (NP_001240812.1) (Pro2-Pro531) was expressed with a polyhistidine tag at the N-terminus.
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Source : Baculovirus-Insect cells
Species : Mouse
Tag : His
Predicted N Terminal : His
Form : Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 5%~8% trehalose and mannitol.
Molecular Mass : The recombinant mouse PKM consists of 548 amino acids and predicts a molecular mass of 60 KDa. It migrates as an approximately 59 KDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Protein length : 2-531 a.a.
Gene Name Pkm pyruvate kinase, muscle [ Mus musculus ]
Official Symbol Pkm
Synonyms Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
Gene ID 18746
mRNA Refseq NM_001253883
Protein Refseq NP_001240812
MIM
UniProt ID P52480
Chromosome Location 9 B; 9 32.03 cM
Pathway Adenine ribonucleotide biosynthesis, IMP =>ADP,ATP, organism-specific biosystem; Biosynthesis of amino acids, organism-specific biosystem; Carbon metabolism, conserved biosystem
Function ADP binding; metal ion binding; poly(A) RNA binding

Not For Human Consumption!

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