Recombinant Mouse Pkm Protein, His-tagged

• Cat.No.: Pkm-968M
• Product Overview: Recombinant mouse PKM (NP_001240812.1) (Pro2-Pro531) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 2-531 a.a.
• Predicted N Terminal: His
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant mouse PKM consists of 548 amino acids and predicts a molecular mass of 60 KDa. It migrates as an approximately 59 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Pkm pyruvate kinase, muscle [ Mus musculus ]
• Official Symbol: Pkm
• Synonyms: Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
• Gene ID: 18746
• mRNA Refseq: NM_001253883
• Protein Refseq: NP_001240812
• UniProt ID: P52480
• Chromosome Location: 9 B; 9 32.03 cM
• Pathway: Adenine ribonucleotide biosynthesis, IMP =>ADP,ATP, organism-specific biosystem; Biosynthesis of amino acids, organism-specific biosystem; Carbon metabolism, conserved biosystem
• Function: ADP binding; metal ion binding; poly(A) RNA binding