Recombinant Mouse Pigf

• Cat.No.: Pigf-330M
• Product Overview: Recombinant mouse PLGF is a disulfide-linked homodimer containing two 142 amino acid polypeptide chains of 16.3 kDa expressed inE. coli.MW=32.6kDa.

Specification

• Cat. No.: Pigf-330M
• Description: Placenta growth factor (PlGF), a homodimeric glycoprotein that is homologous to vascular endothelial growth factor (VEGF), is mitogenic to endothelial cells and protects trophoblast from apoptosis. Alternative splicing of mature mRNA gives rise to three known isoforms of PlGF. PlGF is expressed by human trophoblast during normal pregnancy, however, it is not known which isoforms are produced.
• Source: E. coli.
• Quantity: 10 μg.
• Specific Activity: NA.
• Purity: Greater than 95% (determined by SDS-PAGE and HPLC analysis).
• Endotoxin Level: < 0.1 ng per μg of rMu PLGF-2 (1EU/μg).
• Biological Activity: Determined by its ability to chemoattract monocytes of using a concentration range of 0.5-10 ng/ml.
• Formulation: Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a protein solution with no additives.
• Reconstitution: Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, 20 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
• Storage And Stability: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu PLGF-2 protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: Pigf phosphatidylinositol glycan anchor biosynthesis, class F [ Mus musculus ]
• Synonyms: Pigf; phosphatidylinositol glycan anchor biosynthesis, class F; phosphatidylinositol glycan, class F; OTTMUSP00000029035
• Gene ID: 18701
• mRNA Refseq: NM_008838
• Protein Refseq: NP_032864
• UniProt ID: O09101
• Chromosome Location: 17 E4-E5; 17 54.3 cM
• Pathway: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis; Metabolic pathways
• Function: protein binding; phosphotransferase activity, for other substituted;phosphate groups