Recombinant Mouse Pask Protein, Myc/DDK-tagged

• Cat.No.: Pask-4682M
• Product Overview: Purified recombinant protein of mouse full-length PAS domain containing serine/threonine kinase (Pask), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
• Molecular Mass: 151.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pask PAS domain containing serine/threonine kinase [ Mus musculus (house mouse) ]
• Official Symbol: Pask
• Synonyms: Pask; PAS domain containing serine/threonine kinase; P; mKIAA0135; PAS domain-containing serine/threonine-protein kinase; PAS-kinase; paskin; EC 2.7.11.1
• Gene ID: 269224
• mRNA Refseq: NM_080850
• Protein Refseq: NP_543126
• UniProt ID: Q8CEE6