Recombinant Mouse Parp1 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Parp1-2535MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Parp1 (NP_031441.2) (Met 1-Trp 1014), fused with a polyhistidine tag at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 1033
• Form: Lyophilized
• Molecular Mass: The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly, 0.1 mM TCEP
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Parp1 poly (ADP-ribose) polymerase family, member 1 [ Mus musculus ]
• Official Symbol: Parp1
• Gene ID: 11545
• mRNA Refseq: NM_007415
• Protein Refseq: NP_031441