Recombinant Mouse Pappa Protein, Myc/DDK-tagged

• Cat.No.: Pappa-4673M
• Product Overview: Purified recombinant protein of mouse full-length pregnancy-associated plasma protein A (Pappa), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.
• Molecular Mass: 181.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pappa pregnancy-associated plasma protein A [ Mus musculus (house mouse) ]
• Official Symbol: Pappa
• Synonyms: PAPPA; pregnancy-associated plasma protein A; pappalysin-1; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease; PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik
• Gene ID: 18491
• mRNA Refseq: NM_021362
• Protein Refseq: NP_067337
• UniProt ID: Q8R4K8