Recombinant Human PAPPA Protein, His-tagged

• Cat.No.: PAPPA-2547H
• Product Overview: Recombinant Human PAPPA Protein (Leu317-Pro532) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.3 kDa
  Accurate Molecular Mass: \
• Protein length: Leu317-Pro532
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PAPPA pregnancy-associated plasma protein A, pappalysin 1 [ Homo sapiens (human) ]
• Official Symbol: PAPPA
• Synonyms: PAPPA; pregnancy-associated plasma protein A, pappalysin 1; pappalysin-1; ASBABP2; aspecific BCL2 ARE binding protein 2; differentially placenta 1 expressed protein; DIPLA1; IGFBP 4ase; insulin like growth factor dependent IGF binding protein 4 protease; PAPA; PAPP A; PAPPA1; IGF-dependent IGFBP-4 protease; aspecific BCL2 ARE-binding protein 2; pregnacy-associated plasma protein A; insulin-like growth factor-dependent IGF binding protein-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPP-A; IGFBP-4ase
• Gene ID: 5069
• mRNA Refseq: NM_002581
• Protein Refseq: NP_002572
• MIM: 176385
• UniProt ID: Q13219