Recombinant Mouse Pappa protein, His & GST-tagged

• Cat.No.: Pappa-7723M
• Product Overview: Recombinant Mouse Pappa aa. (Phe999~Asn1272) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: GST&His
• Protein Length: Phe999~Asn1272
• Form: Freeze-dried powder
• Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Pappa pregnancy-associated plasma protein A [ Mus musculus (house mouse) ]
• Official Symbol: Pappa
• Synonyms: PAG1; PAPP-A; IGFBP-4ase; 8430414N03Rik; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF-binding protein 4 protease; pappalysin-1
• Gene ID: 18491
• mRNA Refseq: NM_021362.1
• Protein Refseq: NP_067337.1
• UniProt ID: Q8R4K8