Recombinant Mouse Pacsin1 Protein, Myc/DDK-tagged

• Cat.No.: Pacsin1-4648M
• Product Overview: Purified recombinant protein of mouse full-length protein kinase C and casein kinase substrate in neurons 1 (Pacsin1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission.
• Molecular Mass: 50.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pacsin1 protein kinase C and casein kinase substrate in neurons 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pacsin1
• Synonyms: PACSIN1; protein kinase C and casein kinase substrate in neurons 1; protein kinase C and casein kinase substrate in neurons protein 1; Syndapin I; H74; syndapin; mKIAA1379; A830061D09Rik
• Gene ID: 23969
• mRNA Refseq: NM_011861
• Protein Refseq: NP_035991
• UniProt ID: Q61644