Recombinant Mouse OSTM1 Protein, His-tagged

• Cat.No.: Ostm1-511M
• Product Overview: Recombinant Mouse Ostm1 (NP_766004.1) (Met1-Ser288) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser288
• Description: Osteopetrosis-ass centigradeiated transmembrane protein 1 (OSTM1) is a Single-pass type I membrane protein. It is expressed in many hematopoietic cells of the myeloid and lymphoid B- and T-lineages. The analysis of OSTM1 ass centigradeiation with CLCN7 demonstrated that OSTM1 requires CLCN7 to l centigradealize to lysosomes, whereas the formation of a CLCN7-OSTM1 complex is required to stabilize CLCN7. The researches found that OSTM1 plays a major role in myelopoiesis and lymphopoiesis and provided evidence of a crosstalk mechanism between hematopoietic cells for oste centigradelast activation. Thus, OSTM1 has a important role in oste centigradelast function and activation. The loss of function of OSTM1 results in deregulation of multiple hematopoietic lineages in addition to oste centigradelast lineage, OSTM1-defect patients display the most severe recessive osteopetrotic phenotype and die at early ages. Furthermore, it is suggested that OSTM1 has a primary role in neural development not related to lysosomal dysfunction. The canonical Wnt/beta-catenin signaling pathway may be a molecular basis for OSTM1 mutations and severe autosomal recessive osteopetrosis (ARO).
• Predicted N Terminal: Ala 35
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse Ostm1 consists of 265 amino acids and predicts a molecular mass of 29.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Ostm1 osteopetrosis associated transmembrane protein 1 [ Mus musculus ]
• Official Symbol: Ostm1
• Synonyms: OSTM1; osteopetrosis associated transmembrane protein 1; osteopetrosis-associated transmembrane protein 1; grey lethal osteopetrosis; gl; HSPC019; 1200002H13Rik
• Gene ID: 14628
• mRNA Refseq: NM_172416
• Protein Refseq: NP_766004