Recombinant Mouse Osbp Protein, Myc/DDK-tagged

• Cat.No.: Osbp-4606M
• Product Overview: Purified recombinant protein of mouse full-length oxysterol binding protein (Osbp), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.
• Molecular Mass: 89.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Osbp oxysterol binding protein [ Mus musculus (house mouse) ]
• Official Symbol: Osbp
• Synonyms: OSBP; oxysterol binding protein; oxysterol-binding protein 1; AW559088; mKIAA4220; 1110018F06Rik; KIAA4220
• Gene ID: 76303
• mRNA Refseq: NM_001033174
• Protein Refseq: NP_001028346
• UniProt ID: Q3B7Z2