Recombinant Mouse OPCML protein(Ala5-Val313), His-tagged

• Cat.No.: OPCML-221M
• Product Overview: Recombinant Mouse OPCML (NP_808574.2) (Ala5-Val313) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 5-313 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse OPCmL-His at 10μg/mL (100μL/well) can bind human LSAMP-Fc. The EC50 of human LSAMP-Fc is 0.04-0.1 μg/mL.
• Molecular Mass: The recombinant mouse OPCML comprises 295 amino acids and has a predicted molecular mass of 32.7 kDa. The apparent molecular mass of the protein is approximately 44-55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Opcml opioid binding protein/cell adhesion molecule-like [ Mus musculus ]
• Official Symbol: OPCML
• Synonyms: OPCML; opioid binding protein/cell adhesion molecule-like; Obcam
• Gene ID: 110451