Recombinant Mouse Oga Protein, Myc/DDK-tagged

• Cat.No.: Oga-4578M
• Product Overview: Purified recombinant protein of mouse full-length O-GlcNAcase (Oga), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.
• Molecular Mass: 103.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Oga O-GlcNAcase [ Mus musculus (house mouse) ]
• Official Symbol: Oga
• Synonyms: Oga; O-GlcNAcase; Hy5; Mgea; Mgea5; Ncoat; protein O-GlcNAcase; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; beta-N-acetylhexosaminidase; beta-hexosaminidase; bifunctional protein NCOAT; meningioma expressed antigen 5 (hyaluronidase); meningioma-expressed antigen 5; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; EC 3.2.1.169
• Gene ID: 76055
• mRNA Refseq: NM_023799
• Protein Refseq: NP_076288
• UniProt ID: Q9EQQ9