Recombinant Mouse Nr1i2 Protein, Myc/DDK-tagged

• Cat.No.: Nr1i2-4487M
• Product Overview: Purified recombinant protein of mouse full-length nuclear receptor subfamily 1, group I, member 2 (Nr1i2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Nuclear receptor that binds and is activated by a variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, endogenous compounds and drugs. Response to specific ligands is species-specific, due to differences in the ligand-binding domain. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. Activated by naturally occurring steroids such as pregnenolone and progesterone, the cholesterol metabolite 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol, synthetic glucocorticoids and antiglucocorticoids and 16-alpha-carbonitrile (PCN).
• Molecular Mass: 50 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nr1i2 nuclear receptor subfamily 1, group I, member 2 [ Mus musculus (house mouse) ]
• Official Symbol: Nr1i2
• Synonyms: NR1I2; nuclear receptor subfamily 1, group I, member 2; nuclear receptor subfamily 1 group I member 2; Pregnane X receptor; orphan nuclear receptor PXR; PXR; SXR; PXR1; mPXR; PXR.1; PXR.2
• Gene ID: 18171
• mRNA Refseq: NM_010936
• Protein Refseq: NP_035066
• UniProt ID: O54915