Recombinant Mouse Nprl3 Protein, Myc/DDK-tagged

• Cat.No.: Nprl3-4478M
• Product Overview: Purified recombinant protein of mouse full-length nitrogen permease regulator-like 3 (Nprl3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
• Molecular Mass: 63.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nprl3 nitrogen permease regulator-like 3 [ Mus musculus (house mouse) ]
• Official Symbol: Nprl3
• Synonyms: Nprl3; nitrogen permease regulator-like 3; Ma; Ph; Pr; Aag; Phg; HS-2; HS-4; Mare; HS-26; HS-40; Prox1; m(alp; CGTHBA; m(alpha)RE; GATOR complex protein NPRL3; -14; alpha globin regulatory element containing; alpha-globin regulatory element-containing gene protein; nitrogen permease regulator 3-like protein; proximal locus to the hemoglobin alpha chain complex
• Gene ID: 17168
• mRNA Refseq: NM_181569
• Protein Refseq: NP_853547
• UniProt ID: Q8VIJ8