Recombinant Mouse Nolc1 Protein, Myc/DDK-tagged

• Cat.No.: Nolc1-4457M
• Product Overview: Purified recombinant protein of mouse full-length nucleolar and coiled-body phosphoprotein 1 (Nolc1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.
• Molecular Mass: 73.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nolc1 nucleolar and coiled-body phosphoprotein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Nolc1
• Synonyms: NOLC1; nucleolar and coiled-body phosphoprotein 1; P130; NOPP130; NOPP140; AA408077; AA536818; AU046071; mKIAA0035; 3230402K17Rik
• Gene ID: 70769
• mRNA Refseq: NM_001039352
• Protein Refseq: NP_001034441
• UniProt ID: E9Q5C9