Recombinant Mouse Nog protein, His-tagged, For Organoid Culture

• Cat.No.: Nog-6342M
• Product Overview: Recombinant Mouse Nog protein(P97466)(Gln28-Cys232) is expressed in HEK293 with a 6His tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Gln28-Cys232
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, 5mM EDTA, 5% Trehalose, pH 7.4.
• Bio-activity: Mouse intestinal organoids were cultured with EGF, Noggin and R-spondin 1. After five days of culture, the organoids showed good morphology and germination rate.
• Molecular Mass: 23.9 KDa
• Endotoxin: < 1 EU/μg as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: F2R coagulation factor II (thrombin) receptor [ Homo sapiens ]
• Official Symbol: F2R
• Synonyms: F2R; coagulation factor II (thrombin) receptor; proteinase-activated receptor 1; CF2R; PAR 1; PAR1; TR; thrombin receptor; protease-activated receptor 1; coagulation factor II receptor; HTR; PAR-1
• Gene ID: 2149
• mRNA Refseq: NM_001992
• Protein Refseq: NP_001983
• MIM: 187930
• UniProt ID: P25116