Recombinant Mouse Nit1 Protein, Myc/DDK-tagged

• Cat.No.: Nit1-4419M
• Product Overview: Purified recombinant protein of mouse full-length nitrilase 1 (Nit1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Possesses amidase activity toward deaminated ophthalmate in vitro. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 35.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nit1 nitrilase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Nit1
• Synonyms: NIT1; nitrilase 1; nitrilase homolog 1; ESTM30; W57327; AI255805
• Gene ID: 27045
• mRNA Refseq: NM_012049
• Protein Refseq: NP_036179
• UniProt ID: Q8VDK1