Recombinant Mouse Ngf protein(Ser122-Gly241)

• Cat.No.: Ngf-477M
• Product Overview: Recombinant Mouse NGF (NP_001106168.1) (Ser 122-Gly 241) was expressed in CHO, with an initial Met at the C-terminus.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• ProteinLength: 122-241 a.a.
• Form: Lyophilized from sterile 150mM NaCl, 50mM NaAc, pH 5. 5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL.
• Molecular Mass: The recombinant mouse NGF consists of 121 amino acids and has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ngf nerve growth factor [ Mus musculus ]
• Official Symbol: Ngf
• Synonyms: NGF; nerve growth factor; beta-nerve growth factor; beta-NGF; nerve growth factor, beta; Ngfb
• Gene ID: 18049
• mRNA Refseq: NM_001112698
• Protein Refseq: NP_001106168