Recombinant Mouse Nenf Protein, Myc/DDK-tagged

• Cat.No.: Nenf-4375M
• Product Overview: Purified recombinant protein of mouse full-length neuron derived neurotrophic factor (Nenf), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Acts as a neurotrophic factor in postnatal mature neurons, enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocytes. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance.
• Molecular Mass: 19.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nenf neuron derived neurotrophic factor [ Mus musculus (house mouse) ]
• Official Symbol: Nenf
• Synonyms: NENF; neuron derived neurotrophic factor; neudesin; SCIRP10-related protein; neuron-derived neurotrophic factor; secreted protein of unknown function; Spinal cord injury related protein 10; Spuf; SCIRP10; 1110060M21Rik
• Gene ID: 66208
• mRNA Refseq: NM_025424
• Protein Refseq: NP_079700
• UniProt ID: Q9CQ45