Recombinant Mouse Ndn Protein, Myc/DDK-tagged

• Cat.No.: Ndn-4322M
• Product Overview: Purified recombinant protein of mouse full-length necdin (Ndn), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
• Molecular Mass: 37.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ndn necdin, MAGE family member [ Mus musculus (house mouse) ]
• Official Symbol: Ndn
• Synonyms: NDN; necdin; Peg6; AI528698
• Gene ID: 17984
• mRNA Refseq: NM_010882
• Protein Refseq: NP_035012
• UniProt ID: P25233