Recombinant Mouse Nckipsd Protein, Myc/DDK-tagged

• Cat.No.: Nckipsd-4313M
• Product Overview: Purified recombinant protein of mouse full-length NCK interacting protein with SH3 domain (Nckipsd), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.
• Molecular Mass: 78.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nckipsd NCK interacting protein with SH3 domain [ Mus musculus (house mouse) ]
• Official Symbol: Nckipsd
• Synonyms: NCKIPSD; NCK interacting protein with SH3 domain; NCK-interacting protein with SH3 domain; VIP54; N-WASP-binding protein; SH3 adapter protein SPIN90; mDia interacting protein-1; 54 kDa VacA-interacting protein; 90 kDa N-WASP-interacting protein; WASP-interacting SH3-domain protein; 90 kDa SH3 protein interacting with Nck; Wiskott-Aldrich syndrome homolog binding protein; wiskott-Aldrich syndrome protein-binding protein WISH; DIP1; ORF1; WISH; Wasbp; AF3P21; SPIN90; WASLBP
• Gene ID: 80987
• mRNA Refseq: NM_030729
• Protein Refseq: NP_109654
• UniProt ID: Q9ESJ4