Recombinant Mouse Myl9, His-tagged

• Cat.No.: Myl9-57M
• Product Overview: Recombinant mouse Myl9 was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Myl9 also known as myosin regulatory light polypeptide 9 is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity.
• Form: Liquid. In Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT
• Molecular Mass: 22.4Da (196aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSKRA KAKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DEYLEGMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEASG FIHEDHLREL LTTMGDRFTD EEVDEMYREA PIDKKGNFNY VEFTRILKHG AKDKDD
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Myl9 myosin, light polypeptide 9, regulatory [ Mus musculus ]
• Official Symbol: Myl9
• Synonyms: MYL9; myosin, light polypeptide 9, regulatory; myosin regulatory light polypeptide 9; myosin regulatory light chain 9; myosin light chain, regulatory C; myosin regulatory light chain 2, smooth muscle isoform; MLC20; RLC-C; Mylc2c; AI327049
• Gene ID: 98932
• mRNA Refseq: NM_172118
• Protein Refseq: NP_742116
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Leukocyte transendothelial migration, organism-specific biosystem; Leukocyte transendothelial migration, conserved biosystem; Muscle contraction, organism-specific biosystem
• Function: calcium ion binding