Recombinant Mouse Mydgf Protein

• Cat.No.: Mydgf-01M
• Product Overview: Recombinant Mouse Mydgf Protein without tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was identified in error as interleukin 25. This activity has not been reproducible, however, and the function of this protein is currently unknown.
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Data Not Available.
• Molecular Mass: Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids.
• Protein length: 143 amino acids
• AA Sequence: MVSEPTTVPFDVRPGGVVHSFSQDVGPGNKFTCTFTYASQGGTNEQWQMSLGTSEDSQHFTCTIWRPQGKSYLYFTQFKAELRGAEIEYAMAYSKAAFERESDVPLKSEEFEVTKTAVSHRPGAFKAELSKLVIVAKAARSEL
• Endotoxin: Less than 1 EU/mg of rMuSF20 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 or -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Mydgf myeloid derived growth factor [ Mus musculus (house mouse) ]
• Official Symbol: Mydgf
• Synonyms: Mydgf; myeloid derived growth factor; Il25; Ly6elg; D17Wsu104e; myeloid-derived growth factor; UPF0556 protein C19orf10 homolog
• Gene ID: 28106
• mRNA Refseq: NM_080837
• Protein Refseq: NP_543027
• UniProt ID: Q9CPT4