Recombinant Mouse Mthfd1 Protein, MYC/DDK-tagged

• Cat.No.: Mthfd1-370M
• Product Overview: Purified recombinant protein of full-length mouse methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase (Mthfd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a trifunctional cytoplasmic enzyme. The encoded protein functions as a methylenetetrahydrofolate dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a formyltetrahydrofolate synthase. The encoded enzyme functions in de novo synthesis of purines and thymidylate and in regeneration of methionine from homocysteine.
• Molecular Mass: 101.3 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mthfd1 methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase [ Mus musculus (house mouse) ]
• Official Symbol: Mthfd1
• Synonyms: Mthfd1; methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase; Dcs; Mthfd; E430024A07Rik; C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; C1-tetrahydrofolate synthase; EC 1.5.1.5; EC 3.5.4.9; EC 6.3.4.3
• Gene ID: 108156
• mRNA Refseq: NM_138745
• Protein Refseq: NP_620084
• UniProt ID: Q922D8