Recombinant Mouse Msln protein, His-tagged, Biotinylated(Primary Amine Labeling)

• Cat.No.: Msln-6743M
• Product Overview: Biotinylated Recombinant Mouse Msln protein(Q61468-1)(Asp298-Ser600)(Primary Amine Labeling), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Asp298-Ser600
• Tag: C-His
• Conjugation/Label: Biotin; Primary Amine
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CA125, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.79μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Msln mesothelin [ Mus musculus ]
• Official Symbol: Msln
• Synonyms: MSLN; mesothelin; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; MPF
• Gene ID: 56047
• mRNA Refseq: NM_018857
• Protein Refseq: NP_061345