Recombinant Mouse Mmp9 protein, His-tagged

• Cat.No.: Mmp9-431M
• Product Overview: Recombinant Mouse Mmp9 (P41245) aa. (Ser225~Ser413) fused with His tag was produced in Yeast cells.

Specification

• Species: Mouse
• Source: Yeast
• Tag: His
• Protein Length: Ser225~Ser413
• Description: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.2kDa; Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Mmp9 matrix metallopeptidase 9 [ Mus musculus (house mouse) ]
• Official Symbol: Mmp9
• Synonyms: Mmp9; matrix metallopeptidase 9; Clg4b; Gel B; MMP-9; B/MMP9; AW743869; pro-MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; collagenase type IVB; gelatinase B
• Gene ID: 17395
• mRNA Refseq: NM_013599.4
• Protein Refseq: NP_038627.1
• UniProt ID: P41245