Recombinant Mouse Mif protein, His-tagged

• Cat.No.: Mif-4001M
• Product Overview: Recombinant Mouse Mif protein(P34884)(Pro2-Ala115), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Pro2-Ala115
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The secreted recombinant mouse MIF consists of 125 amino acids and has a calculated molecular mass of 13.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 18 and 22 kDa in SDS-PAGE under reducing conditions due to different glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Mif macrophage migration inhibitory factor [ Mus musculus ]
• Official Symbol: Mif
• Synonyms: MIF; macrophage migration inhibitory factor; DER6; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; glycosylation-inhibiting factor; delayed early response protein 6; GIF; Glif; MGC107654
• Gene ID: 17319
• mRNA Refseq: NM_010798
• Protein Refseq: NP_034928