Recombinant Mouse Mid1 protein, His & T7-tagged

• Cat.No.: Mid1-7951M
• Product Overview: Recombinant Mouse Mid1 aa. (Met1~Leu212 (Accession # O70583)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&T7
• Protein Length: Met1~Leu212
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.5kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Mid1 midline 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mid1
• Synonyms: Mid1; midline 1; Fxy; 61B3-R; Trim18; DXHXS1141; E3 ubiquitin-protein ligase Midline-1; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; midin; tripartite motif-containing protein 18
• Gene ID: 17318
• mRNA Refseq: NM_001290504.1
• Protein Refseq: NP_001277433.1
• UniProt ID: O70583