Recombinant Mouse Med10 Protein, Myc/DDK-tagged

• Cat.No.: Med10-4012M
• Product Overview: Purified recombinant protein of mouse full-length mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S. cerevisiae) (cDNA clone MGC:66801, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
• Molecular Mass: 15.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Med10 mediator complex subunit 10 [ Mus musculus (house mouse) ]
• Official Symbol: Med10
• Synonyms: Med10; mediator complex subunit 10; C78613; AA959813; AI385605; D13Wsu50; D13Wsu50e; mediator of RNA polymerase II transcription subunit 10; mediator of RNA polymerase II transcription, subunit 10 homolog (NUT2, S. cerevisiae)
• Gene ID: 28077
• mRNA Refseq: NM_138596
• Protein Refseq: NP_613062
• UniProt ID: Q9CXU0