Recombinant Mouse Mdm2 Protein, His-tagged

• Cat.No.: Mdm2-1771M
• Product Overview: Recombinant Mouse Mdm2 Protein (Met1-Asn310) with N-His tag was expressed in E. coli.

Specification

• Description: Enables p53 binding activity and ubiquitin protein ligase activity. Involved in apoptotic process and positive regulation of muscle cell differentiation. Acts upstream of or within several processes, including blood vessel remodeling; circulatory system development; and traversing start control point of mitotic cell cycle. Located in cytoplasm and nucleolus. Is expressed in several structures, including alimentary system; embryo ectoderm; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in chronic myeloid leukemia; endocrine gland cancer (multiple); glioblastoma; ichthyosis; and sarcoma. Orthologous to human MDM2 (MDM2 proto-oncogene).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.2 kDa
• Protein length: Met1-Asn310
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Mdm2 transformed mouse 3T3 cell double minute 2 [ Mus musculus (house mouse) ]
• Official Symbol: Mdm2
• Synonyms: MDM2; transformed mouse 3T3 cell double minute 2; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; double minute 2 protein; p53-binding protein Mdm2; Mdm-2; AA415488; 1700007J15Rik
• Gene ID: 17246
• mRNA Refseq: NM_010786
• Protein Refseq: NP_034916
• UniProt ID: P23804