Recombinant Mouse Mcm7 Protein, Myc/DDK-tagged

• Cat.No.: Mcm7-3998M
• Product Overview: Purified recombinant protein of mouse full-length minichromosome maintenance complex component 7 (Mcm7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
• Molecular Mass: 81.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mcm7 minichromosome maintenance complex component 7 [ Mus musculus (house mouse) ]
• Official Symbol: Mcm7
• Synonyms: MCM7; minichromosome maintenance deficient 7 (S. cerevisiae); DNA replication licensing factor MCM7; CDC47 homolog; mini chromosome maintenance deficient 7; Mcmd7; mCDC47; AI747533
• Gene ID: 17220
• mRNA Refseq: NM_008568
• Protein Refseq: NP_032594
• UniProt ID: Q61881