Recombinant Mouse Map2k1 Protein, Gly/Pro-tagged, Alexa Fluor 555 conjugated

• Cat.No.: MAP2K1-483MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse MAP2K1 (NP_032953.1) (Met1-Ile393), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Protein Length: 395
• Form: Lyophilized
• Molecular Mass: The recombinant mouse MAP2K1 consists of 395 amino acids and predicts a molecular mass of 43.6 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Gly
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Map2k1 mitogen-activated protein kinase kinase 1 [ Mus musculus ]
• Official Symbol: MAP2K1
• Gene ID: 26395
• mRNA Refseq: NM_008927
• Protein Refseq: NP_032953