Recombinant Mouse MANF Protein, His-tagged

• Cat.No.: MANF-935M
• Product Overview: Recombinant mouse MANF (NP_083379.2) (Met 1-Leu 179) was expressed, with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• ProteinLength: 1-179 a.a.
• Predicted N Terminal: Leu 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant mouse MANF comprises 169 amino acids and has a calculated molecular mass of 19.6 kDa. The apparent molecular mass of the recombinant protein is approximately 21 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Manf mesencephalic astrocyte-derived neurotrophic factor [ Mus musculus ]
• Official Symbol: MANF
• Synonyms: MANF; mesencephalic astrocyte-derived neurotrophic factor; arginine-rich protein; arginine-rich, mutated in early stage tumors; Armet; D17914; AA407711; AA408789; AA673178; D18Mgi17; 3230402M22Rik
• Gene ID: 74840
• mRNA Refseq: NM_029103
• Protein Refseq: NP_083379