Recombinant Mouse Lpcat2 Protein, Myc/DDK-tagged

• Cat.No.: Lpcat2-3808M
• Product Overview: Purified recombinant protein of mouse full-length lysophosphatidylcholine acyltransferase 2 (Lpcat2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Exhibits both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Involved in the regulation of lipid droplet number and size.
• Molecular Mass: 60.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Lpcat2 lysophosphatidylcholine acyltransferase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Lpcat2
• Synonyms: LPCAT2; lysophosphatidylcholine acyltransferase 2; LPCAT-2; lysoPAFAT; lysoPAFAT/LPCAT2; LPC acyltransferase 2; acyltransferase like 1; acyltransferase-like 1; acyltransferase like 1A; lysoPC acyltransferase 2; lyso-PAF acetyltransferase; acetyl-CoA:lyso-PAF acetyltransferase; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase member 1; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Aytl1; Aytl1a; lpafat1; A330042H22
• Gene ID: 270084
• mRNA Refseq: NM_173014
• Protein Refseq: NP_766602
• UniProt ID: Q8BYI6