Recombinant Mouse Limch1 Protein, Myc/DDK-tagged

• Cat.No.: Limch1-3782M
• Product Overview: Purified recombinant protein of mouse full-length LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.
• Molecular Mass: 100.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Limch1 LIM and calponin homology domains 1 [ Mus musculus (house mouse) ]
• Official Symbol: Limch1
• Synonyms: Limch1; LIM and calponin homology domains 1; mKIAA1102; 3732412D22Rik; LIM and calponin homology domains-containing protein 1
• Gene ID: 77569
• mRNA Refseq: NM_001001980
• Protein Refseq: NP_001001980
• UniProt ID: Q3UH68