Recombinant Mouse Lilrb4a protein, His-tagged

• Cat.No.: Lilrb4a-0626M
• Product Overview: Recombinant Mouse Lilrb4a protein(Q64281-1)(Gly24-Lys238), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• ProteinLength: Gly24-Lys238
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.