Recombinant Mouse Lif Protein

• Cat.No.: Lif-25M
• Product Overview: Recombinant mouse LIF protein without tag was expressed in E. coli.
  Animal-free (AOF) and carrier protein-free.
  Mass spectrometry: single species with expected mass.
  Recovery from stock vial: >95%

Specification

• Species: Mouse
• Source: E.coli
• Description: Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).
• Molecular Mass: 20 kDa
• Endotoxin: <0.005 EU/μg protein (below level of detection)
• Applications: Differentiation of embryonic Stem cells into gut-like structures, cardiomyocytes and hepatocytes; epithelial to mesenchymal transition
• Storage: Resuspend in 10mM HCl at >100 μg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade
• Storage Buffer: Lyophilized from acetonitrile, TFA

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Mus musculus (house mouse) ]
• Official Symbol: Lif
• Synonyms: Lif; leukemia inhibitory factor; leukemia inhibitory factor; d factor; differentiation-stimulating factor; myeloid leukaemia inhibitory factor
• Gene ID: 16878
• mRNA Refseq: NM_008501
• Protein Refseq: NP_032527
• UniProt ID: P09056