Recombinant Mouse Lacc1 Protein, Myc/DDK-tagged

• Cat.No.: Lacc1-3746M
• Product Overview: Purified recombinant protein of mouse full-length laccase domain containing 1 (Lacc1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Purine nucleoside enzyme that catalyzes the phosphorolysis of adenosine, guanosine and inosine nucleosides, yielding D-ribose 1-phosphate and the respective free bases, adenine, guanine and hypoxanthine. Also catalyzes the phosphorolysis of S-methyl-5'-thioadenosine into adenine and S-methyl-5-thio-alpha-D-ribose 1-phosphate. Also has adenosine deaminase activity. Acts as a regulator of innate immunity in macrophages by modulating the purine nucleotide metabolism, thereby regulating the metabolic function and bioenergetic state of macrophages. Enables a purine nucleotide cycle between adenosine and inosine monophosphate and adenylosuccinate that prevents cytoplasmic acidification and balances the cytoplasmic-mitochondrial redox interface. The purine nucleotide cycle consumes aspartate and releases fumarate in a manner involving fatty acid oxidation and ATP-citrate lyase activity. Participates in pattern recognition receptor-induced cytokines in macrophages: associates with the NOD2-signaling complex and promotes optimal NOD2-induced signaling, cytokine secretion and bacterial clearance. Localizes to the endoplasmic reticulum upon PRR stimulation of macrophages and associates with endoplasmic reticulum-stress sensors, promoting the endoplasmic reticulum unfolded protein response (UPR). Does not show laccase activity.
• Molecular Mass: 48 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Lacc1 laccase domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Lacc1
• Synonyms: Lacc1; laccase domain containing 1; 9030625A04Rik; purine nucleoside phosphorylase LACC1; S-methyl-5'-thioadenosine phosphorylase LACC1; adenosine deaminase LACC1; fatty acid metabolism-immunity nexus; guanosine phosphorylase LACC1; laccase (multicopper oxidoreductase) domain containing 1; laccase domain-containing protein 1; EC 2.4.2.1; EC 2.4.2.28; EC 3.5.4.4
• Gene ID: 210808
• mRNA Refseq: NM_172488
• Protein Refseq: NP_766076
• UniProt ID: Q8BZT9