Recombinant Mouse Kel Protein, His-tagged

• Cat.No.: Kel-018M
• Product Overview: Recombinant mouse Kell, fused to His-tag at C terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect cells
• Tag: His
• Description: Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3.
• Form: Liquid
• Molecular Mass: 76.3 kDa
• AA Sequence: IFRNCGPCPCETPVCMELLDHYLASGNRSVAPCTDFFSFACEKANGTSDSFQALTEENKSRLWRLLEAPGSWHLGSGEEKAFQFYNSCMDTDAIEASGSGPLIQIIEELGGWNITGNWTSLDFNQNLRLLMSQYGHFPFFRAYLRPHPAPPHTPIIQIDQPEFDILLQQEQEQKVYAQILREYVTYLNRLGTLLGSNPQEAQQHASWSIVFTSRLFQFLRPQQQQQAQDKLFHVVTIDELQEMAPAIDWLSCLQAIFTPMSLNSSQTLVVHDLDYLRNMSQLVEEGLLNHRESIQSYMILGLVDTLSPALDTKFQEARRELIQELRKLKERPPLPAYPRWMKCVEQTGAFFEPTLAALFVREAFGPSIQSAAMELFAEIKDAVIIRLKKLSWISEETQKEALNKLAQLQVEMGAPKRAVKPDIATQEYNDIQLGPSFLQSFLSCVRSLRARNVQSFLQPFPYHRWQKSPWEVNAYYSISDHMVVFPAGLLQPPFFHPGYPRAVNFGAAGSIMAHELLHIFYQLLLPGGCPACDTHVLQEALLCLERHYAAFPLPSISSFNGSHTLLENAADIGGVAIAFQAYSKRIVEHTGELTLPNLDLSPYQLFFRSYAQVMCRGLSSQDPQDPHSPPSLRVHGPLSNTPDFAKHFHCPRGTLLNPSARCKLW
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -97 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Kel Kell blood group [ Mus musculus (house mouse) ]
• Official Symbol: Kel
• Synonyms: Kel; Kell blood group; Ece3; CD238; kell blood group glycoprotein homolog; Kel blood group protein; Kell blood group, metallo-endopeptidase; Kell protein
• Gene ID: 23925
• mRNA Refseq: NM_032540
• Protein Refseq: NP_115929
• UniProt ID: Q9EQF2