Recombinant Mouse Itga3 protein, His & T7-tagged

• Cat.No.: Itga3-6773M
• Product Overview: Recombinant Mouse Itga3 aa. (Phe33~Glu236 (Accession # Q62470)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&T7
• Protein Length: Phe33~Glu236
• Description: This gene encodes a subunit of integrin family of cell surface proteins. The encoded protein undergoes post-translational processing to form a disulfide bond-linked dimer comprised of heavy and light chains. At the cell surface, the encoded protein non-covalently associates with the integrin beta-1 subunit to form a heterodimer that interacts with many extracellular matrix proteins including fibronectin and laminin. Mice lacking the encoded protein die during the first day after birth due to severe abnormalities in kidneys. Mice lacking the encoded protein specifically in the basal layer of epidermis display several skin defects and accelerated wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.8kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Itga3 integrin alpha 3 [ Mus musculus (house mouse) ]
• Official Symbol: Itga3
• Synonyms: CD49C; GAPB3; AA407068; CD49 antigen-like family member C; VLA-3 receptor, alpha 3 subunit; galactoprotein B3; integrin alpha-3
• Gene ID: 16400
• mRNA Refseq: NM_001306071.1
• Protein Refseq: NP_001293000.1
• UniProt ID: Q62470