Recombinant Mouse Ipo11 Protein, Myc/DDK-tagged

• Cat.No.: Ipo11-3564M
• Product Overview: Purified recombinant protein of mouse full-length importin 11 (Ipo11), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPL12, and of UBE2E3.
• Molecular Mass: 112.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ipo11 importin 11 [ Mus musculus (house mouse) ]
• Official Symbol: Ipo11
• Synonyms: IPO11; importin 11; importin-11; imp11; ran-binding protein 11; Ranbp11; AI314624; AW555235; 1700081H05Rik; 2510001A17Rik; E330021B14Rik
• Gene ID: 76582
• mRNA Refseq: NM_029665
• Protein Refseq: NP_083941
• UniProt ID: Q8K2V6