Recombinant Mouse Interleukin 4 Receptor, Alpha, Fc-tagged

• Cat.No.: Il4ra-1757M
• Product Overview: Recombinant Mouse Il4ra encoding the extracellular domain (amino acid residues 26 - 233) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide. The chimeric protein was expressed in a mouse myeloma cell line,NSO.

Specification

• Cat. No.: Il4ra-1757M
• Description: Interleukin 4 receptor is a type I cytokine receptor. IL4R is its human gene. This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. Among T cells, the encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse IL-4 R is Mr 51 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 65-80 kDa.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Endotoxin: <0.1 ng per 1 µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse IL-4 R was determined by its ability to inhibit the IL-4-dependent proliferation of HT2 cells. The expected ED50 for this effect is typically 0.05-0.2 μg/ml in the presence of 3 ng/ml of Mouse IL-4.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
• Protein length: 26-233 a.a.

Gene Information

• Gene Name: Il4ra interleukin 4 receptor, alpha [ Mus musculus ]
• Synonyms: Il4ra; interleukin 4 receptor, alpha; Il4r; CD124; MGC118473;IL-4 receptor alpha chain
• Gene ID: 16190
• mRNA Refseq: NM_001008700
• Protein Refseq: NP_001008700
• UniProt ID: P16382
• Chromosome Location: 7 F3; 7 62.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
• Function: cytokine receptor activity; protein binding; receptor activity