Recombinant Mouse Interleukin 27

• Cat.No.: Il27-75M
• Product Overview: Recombinant Mouse Interleukin-27 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23.7 kDa. The Murine IL-27 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Il27-75M
• Description: Interleukin-27 protein is related to IL-12A & is one of the subunits of a heterodimeric cytokine complex. IL-27 interacts with EBV induced gene 3 also called EBI3, and forms a complex that drives rapid expansion of CD4 (+) T cells. IL-27 complex is synergizes with IL-12 in order to trigger the cytokine production of IFN-Gamma of CD4 (+) T cells. The biological effect of IL-27 is mediated by class-I cytokine receptor (WSX1/TCRR). The pro-inflammatory activity of IL-27 is mediated through the growing expression of key molecules involved in the MHC class-I & MHC class-II pathways. Both MHC class-I and MHC-class-II expression are increased in endothelial cells after Interleukin-27 stimulation which suggests that it may play an important role in conferring the immune function on vascular endothelium IL-27p28 subunit can be induced by IFN-beta and during LPS-induced maturation of dendritic cells in type-I IFN-dependent manner through IFN regulatory factor-1 activation. Interleukin-27 regulates Interleukin-12 responsiveness of CD4+ T cells through Stat1-dependent and -independent mechanisms. IL-17 & IL-23 play an important IL-17 role in inflammation. Interleukin-27 possesses potent anti-angiogenic activity that plays an important role in its antitumor and antimetastatic activities. EBV induced gene 3 plays a role, independently from IL-27, in regulating anti-viral or anti-tumoral immune responses. Interleukin-27 is a potent inhibitor of HIV-1 replication in macrophages, CD4+ T cells, peripheral blood mononuclear cells. Interleukin-27 triggers STAT activation and gene transcription.
• Source: Escherichia Coli.
• Amino Acid Sequence: MFP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHLSDSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLP GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from 10mM sodium phosphate buffer pH-7.5.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-27 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized Interleukin-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus ]
• Synonyms: Il27; interleukin 27; p28; Il30; IL-27; IL-27p28; interleukin 30 ; IL-27 p28 subunit
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611
• UniProt ID: Q8K3I6
• Chromosome Location: 7 F3
• Function: cytokine activity; interleukin-27 receptor binding; protein binding; receptor binding