Recombinant Mouse Interleukin 18, His-tagged
Cat.No. : | Il18-740M |
Product Overview : | Recombinant Mouse Il18 was expressed as His-tagged fusion protein in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW: 20.4 kDa. |
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Species : | Mouse |
Source : | E.coli |
Tag : | His |
Protein Length : | 36-192aa |
Description : | Il18, previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
Form : | In 20mM Tris-HCl pH 8.0 containing 10% glycerol |
Molecular Mass : | 20.4 kDa (178 aa), confirmed by MALDI-TOF. |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS |
Endotoxin : | < 1.0 eu per 1 μg of protein (determined by lal |
Purity : | >90% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Gene Name | Il18 interleukin 18 [ Mus musculus ] |
Official Symbol | Il18 |
Synonyms | IL18; interleukin 18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Igif; Il-18; |
Gene ID | 16173 |
mRNA Refseq | NM_008360 |
Protein Refseq | NP_032386 |
Pathway | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Hypertrophy Model, organism-specific biosystem; |
Function | cytokine activity; |
◆ Recombinant Proteins | ||
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Il18-5439M | Recombinant Mouse Il18 protein, His-tagged | +Inquiry |
IL18-3031R | Recombinant Rat IL18 Protein | +Inquiry |
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◆ Cell & Tissue Lysates | ||
IL18-344HCL | Recombinant Human IL18 lysate | +Inquiry |
Not For Human Consumption!
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Q&As (1)
Ask a questionIL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.
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